生物成像

生物成像

深层组织、
多模态和共焦成像的革新

  • 最宽广的调谐范围,最短的超快脉冲、可完成最深层的活体成像
  • 已通过拥有最大客户群获得证实
  • 高度可靠、多年稳定保持正常工作

Bio-Imaging

Spectra-Physics 是生物成像激光器的市场领导者. 凭借最新推出的超一流 InSight X3 超快激光器、Spectra-Physics 的创新将多光子成像推向新高度、再次推动技术进步. 在多种要求严格的生物成像应用中、已部署了几千个 Spectra-Physics 激光器、其作为可靠解决方案已得到完美证实。

联系我们的专家、讨论您激光应用的相关需求和挑战. 让我们通力合作、解决您的难题

Insight DeepSee ultrafast laser

InSight X3

Widely tunable ultrafast laser system for multiphoton images

new

生物成像应用

Mouse brain, hippocampus region; imaged with femtoTrain™.
Courtesy of Sabine Scheibe, LMU Munich and Tilman Franke, FEI Munich GmbH



Mouse barrel cortex, after clearing.
Courtesy of Gabriel Jones, Steven Petrou, University of Melbourne, Australia



Hippocampus CA3 pyramidal neuron; imaged with InSight® DS+™.
Courtesy of Karina Alvina, Albert Einstein College of Medicine



Multicolor image of live mouse brain; imaged with InSight DS+.
Courtesy of Naoki Honkura and Takeshi Imamura, Ehime University Graduate School of Medicine



Zebrafish embryo brain development, CFP, YFP, mCherry imaged at 860 nm and 1041 nm with InSight® DS+™.
Courtesy of Dr. Nadine Peyrieras, CNRS, Gif sur Yvette, France and LaVision Biotec



Maximum intensity projection of zebrafish embryo development, taken from both sides over 8 hours; imaged with InSight DS+.
Courtesy of Dr. Nadine Peyrieras, CNRS, Gif sur Yvette, France and LaVision Biotec



Z-stack of transgenic zebrafish larvae, labeled with GFP and CFP; imaged with Mai Tai® DeepSee™.
Courtesy of Dr. Rachel Wong, University of Washington, Seattle



Transgenic zebrafish embryo retina, revealing various neuronal cell types, imaged at 927 nm and 1041 nm with InSight DS+.
Courtesy of Dr. Xana Almeida, University of Cambridge, UK



Lymph vessel; imaged with InSight® DS+™.
Courtesy of Dr. Cameron Newell, Monash, University, Melbourne, Australia



Continuous tracking of T cells (tagged with CMPTX, imaged at 1080 nm) and dendritic cells
(tagged with GFP, imaged at 925 nm) interacting in mouse skin over several hours; imaged with InSight DS+.
Courtesy of Dr. Michael Kuligowski, Centenary Institute of Cancer Research and Cell Biology, Sydney, Australia



Continuous tracking of T cells (tagged with CMPTX, imaged at 1080 nm) and dendritic cells
(tagged with GFP, imaged at 925 nm) interacting in mouse skin over several hours; imaged with InSight DS+.
Courtesy of Dr. Michael Kuligowski, Centenary Institute of Cancer Research and Cell Biology, Sydney, Australia



Mouse ear, showing collagen (blue) and GFP labelled T cells (green); imaged with Mai Tai® DeepSee™.
Courtesy of Dr. Claudio Vinegoni, Mass General Hospital, Harvard University



Retinal ganglion cell; imaged with Mai Tai.
Courtesy of Dr. Gregory Schwartz, Northwestern University



Chemically cleared mouse heart total depth of 3.5 mm; imaged with Mai Tai DeepSee.
Courtesy of Mayandi Sivaguru, University of Illinois Urbana Champaign and Sakthivel Sadayappan, Loyola University, Chicago



Human meibomian gland, CARS imaging the lipid rich meibocytes and SHG visualizing the surrounding collagen;
acquired with InSight® DS+™.
Courtesy of Dr. Eric Potma, UC Irvine



SRS image of fatty liver, pump 802 nm and Stokes at 1040 nm; acquired with InSight DS+.
Courtesy of Dr. Ji-XIn Cheng, Purdue University



SRS image of spinal cord, pump 802 nm and Stokes at 1040 nm; acquired with InSight DS+.
Courtesy of Dr. Ji-XIn Cheng, Purdue University



CARS Z-stack of C.Elegans worm, visualizing lipids 19.
Courtesy of Dr. Daewon Moon and Dr. Hyunmin Kim, DGIST Daegu Gyeongbuk Institute of Science and Technology (DGIST)



CARS and MPEF imaging of C. Elegans, neuron cells labelled with GFP, lipid droplets revealed with CARS.
Courtesy of Dr. Daewon Moon and Dr. Hyunmin Kim, DGIST Daegu Gyeongbuk Institute of Science and Technology (DGIST)



Zebrafish embryo (6 hours post fertilization) using label free THG at 1140 nm; imaged with InSight® DS+™.
Courtesy of Dr. Nadine Peyrieras, CNRS, Gif sur Yvette, France



Mouse blood vessel wall, SHG and multiphoton excited fluorescence imaging with HighQ-2™.
Courtesy of Dr. Marc van Zandvoort, Maastrich University



Mouse mammary gland, label free image of collagen (SHG, magenta) and adipocytes (THG, yellow), imaged with InSight DS+™.
Courtesy of Dr. Marie Irondelle, Institut Curie/CNRS, Paris, France



3D images from a mouse brain cerebellum extending 1 mm deep into the tissue acquired via 3PF (left)
and THG (right) microscopy at 1.3 μm using a Spirit laser with a Spirit-NOPA.
Courtesy of Chris Xu, with permission from SPIE Publications: Wang, et. al., “In vivo three-photon
imaging of deep cerebellum,” Proc. SPIE:  Multiphoton Microscopy in the Biomedical Sciences
XVIII, vol. 10498, 2018.

Mouse kidney, with CARS (red), SHG (blue) and autofluorescence (green); imaged with Mai Tai® HP plus Inspire™ OPO.
Courtesy of Dr. Eric Potma, UC Irvine, CA



C. elegans, with CARS mapping out lipid rich areas and two-photon excited fluorescence from GFP and dsRed;
imaged with InSight® DS+™.
Courtesy of Dr. Eric Potma, UC Irvine, CA

生物成像激光器

产品类型波长脉冲宽度
new
InSight X3
超宽调谐、内置色散补偿、多光子成像用飞秒激光器
超快 680–1300 nm <120 fs  
Mai Tai DeepSee
宽广调谐范围,内置色散补偿、可保证最高峰值功率
690–1040 nm 70–100 fs  
Mai Tai
宽广调谐范围和超短脉冲宽度
690–1040 nm 70–100 fs  
new
Spirit
设定高精度飞秒加工的新标准
515, 520, 1030, 1040 nm 400 fs  
HighQ-2
一体式固定波长飞秒激光器
Fixed, 1045 nm <250 fs  
femtoTrain
一体式高功率飞秒激光器
Fixed, 1040 nm <220 fs  
Inspire
光学参数振荡器、具有从紫外到红外的超宽光谱范围
345–2500 nm 80–250 fs  
Excelsior One
紧凑轻便的紫外纳秒激光器
CW 375、405、445、473、488、532、542、561、
642、785、and 1064 nm
不适用  
Excelsior
紧凑型 CW 激光器(科学和 OEM)
375、405、440、473、488、505、515、532、542、561、
594、642、785、and 1064 nm