bio-imaging

bio-imaging

Revolutionizing Deep Tissue,
Multimodal and Confocal Imaging

  • enabling deepest in vivo imaging with broadest tuning, shortest pulse ultrafast
  • proven with largest installed base
  • delivering highest reliability for dependable multi-year operation

Bio-Imaging

Spectra-Physics is a market leader in lasers for bio-imaging. With the introduction of the extraordinary InSight X3 ultrafast lasers, Spectra-Physics innovations again advance the state-of-the-art by taking multiphoton imaging to new depths. With thousands of lasers deployed in demanding bio-imaging applications, Spectra-Physics lasers are a proven, reliable solution.

So contact our experts to discuss your laser application needs and challenges. Let’s work together to solve them.

 

Insight DeepSee ultrafast laser

InSight X3

Widely tunable ultrafast laser system for multiphoton images

new

Bio-Imaging Applications

Mouse brain, hippocampus region; imaged with femtoTrain™.
Courtesy of Sabine Scheibe, LMU Munich and Tilman Franke, FEI Munich GmbH



Mouse barrel cortex, after clearing.
Courtesy of Gabriel Jones, Steven Petrou, University of Melbourne, Australia



Hippocampus CA3 pyramidal neuron; imaged with InSight® DS+™.
Courtesy of Karina Alvina, Albert Einstein College of Medicine



Multicolor image of live mouse brain; imaged with InSight DS+.
Courtesy of Naoki Honkura and Takeshi Imamura, Ehime University Graduate School of Medicine



Zebrafish embryo brain development, CFP, YFP, mCherry imaged at 860 nm and 1041 nm with InSight® DS+™.
Courtesy of Dr. Nadine Peyrieras, CNRS, Gif sur Yvette, France and LaVision Biotec



Maximum intensity projection of zebrafish embryo development, taken from both sides over 8 hours; imaged with InSight DS+.
Courtesy of Dr. Nadine Peyrieras, CNRS, Gif sur Yvette, France and LaVision Biotec



Z-stack of transgenic zebrafish larvae, labeled with GFP and CFP; imaged with Mai Tai® DeepSee™.
Courtesy of Dr. Rachel Wong, University of Washington, Seattle



Transgenic zebrafish embryo retina, revealing various neuronal cell types, imaged at 927 nm and 1041 nm with InSight DS+.
Courtesy of Dr. Xana Almeida, University of Cambridge, UK



Lymph vessel; imaged with InSight® DS+™.
Courtesy of Dr. Cameron Newell, Monash, University, Melbourne, Australia



Continuous tracking of T cells (tagged with CMPTX, imaged at 1080 nm) and dendritic cells
(tagged with GFP, imaged at 925 nm) interacting in mouse skin over several hours; imaged with InSight DS+.
Courtesy of Dr. Michael Kuligowski, Centenary Institute of Cancer Research and Cell Biology, Sydney, Australia



Continuous tracking of T cells (tagged with CMPTX, imaged at 1080 nm) and dendritic cells
(tagged with GFP, imaged at 925 nm) interacting in mouse skin over several hours; imaged with InSight DS+.
Courtesy of Dr. Michael Kuligowski, Centenary Institute of Cancer Research and Cell Biology, Sydney, Australia



Mouse ear, showing collagen (blue) and GFP labelled T cells (green); imaged with Mai Tai® DeepSee™.
Courtesy of Dr. Claudio Vinegoni, Mass General Hospital, Harvard University



Retinal ganglion cell; imaged with Mai Tai.
Courtesy of Dr. Gregory Schwartz, Northwestern University



Chemically cleared mouse heart total depth of 3.5 mm; imaged with Mai Tai DeepSee.
Courtesy of Mayandi Sivaguru, University of Illinois Urbana Champaign and Sakthivel Sadayappan, Loyola University, Chicago



Human meibomian gland, CARS imaging the lipid rich meibocytes and SHG visualizing the surrounding collagen;
acquired with InSight® DS+™.
Courtesy of Dr. Eric Potma, UC Irvine



SRS image of fatty liver, pump 802 nm and Stokes at 1040 nm; acquired with InSight DS+.
Courtesy of Dr. Ji-XIn Cheng, Purdue University



SRS image of spinal cord, pump 802 nm and Stokes at 1040 nm; acquired with InSight DS+.
Courtesy of Dr. Ji-XIn Cheng, Purdue University



CARS Z-stack of C.Elegans worm, visualizing lipids 19.
Courtesy of Dr. Daewon Moon and Dr. Hyunmin Kim, DGIST Daegu Gyeongbuk Institute of Science and Technology (DGIST)



CARS and MPEF imaging of C. Elegans, neuron cells labelled with GFP, lipid droplets revealed with CARS.
Courtesy of Dr. Daewon Moon and Dr. Hyunmin Kim, DGIST Daegu Gyeongbuk Institute of Science and Technology (DGIST)



Zebrafish embryo (6 hours post fertilization) using label free THG at 1140 nm; imaged with InSight® DS+™.
Courtesy of Dr. Nadine Peyrieras, CNRS, Gif sur Yvette, France



Mouse blood vessel wall, SHG and multiphoton excited fluorescence imaging with HighQ-2™.
Courtesy of Dr. Marc van Zandvoort, Maastrich University



Mouse mammary gland, label free image of collagen (SHG, magenta) and adipocytes (THG, yellow), imaged with InSight DS+™.
Courtesy of Dr. Marie Irondelle, Institut Curie/CNRS, Paris, France



3D images from a mouse brain cerebellum extending 1 mm deep into the tissue acquired via 3PF (left)
and THG (right) microscopy at 1.3 μm using a Spirit laser with a Spirit-NOPA.
Courtesy of Chris Xu, with permission from SPIE Publications: Wang, et. al., “In vivo three-photon
imaging of deep cerebellum,” Proc. SPIE:  Multiphoton Microscopy in the Biomedical Sciences
XVIII, vol. 10498, 2018.

C. elegans, with CARS mapping out lipid rich areas and two-photon excited fluorescence from GFP and dsRed;
imaged with InSight® DS+™.
Courtesy of Dr. Eric Potma, UC Irvine, CA

Mouse kidney, with CARS (red), SHG (blue) and autofluorescence (green); imaged with Mai Tai® HP plus Inspire™ OPO.
Courtesy of Dr. Eric Potma, UC Irvine, CA

Lasers for Bio-Imaging

ProductTypeWavelengthPulse Width
new
InSight X3
Widely tunable ultrafast laser system for multiphoton images
Ultrafast 680–1300 nm <120 fs  
Mai Tai DeepSee
Broad tuning range, built-in dispersion compensation for highest peak power
690–1040 nm 70–100 fs  
Mai Tai
Broad tuning range and ultra short pulse width
690–1040 nm 70–100 fs  
new
Spirit
Setting a new standard for high-precision femtosecond machining
515, 520, 1030, 1040 nm 400 fs  
HighQ-2
Ultra-compact turn-key single wavelength femtosecond lasers
Fixed, 1045 nm <250 fs  
femtoTrain
Compact turn-key high energy femtosecond lasers
Fixed, 1040 nm <220 fs  
Inspire
Optical parametric oscillators with ultra-wide spectral coverage from UV to IR
345–2500 nm 80–250 fs  
Excelsior One
Compact all-in-one CW lasers (OEM only)
CW 375, 405, 445, 473, 488, 532, 542, 561,
642, 785, and 1064 nm
N/A  
Excelsior
Compact CW lasers (scientific and OEM)
375, 405, 440, 473, 488, 505, 515, 532, 542, 561,
594, 642, 785, and 1064 nm
 

Application Notes

Two-Photon Excited Fluorescence Microscopy
CARS Imaging Complements Other Multimodal Imaging Techniques
Computer Controlled Variable Attenuator for Tunable Lasers
Variable Attenuator for Lasers

Technical Articles

Resource Library